Consistent readings mean the HPLC pump system is Measurement accuracy is ☑ percent of the reading over flow LCD panel and recorded to a six-digit printer or computer. Readings are displayed on the meter’s four-digit.Timer stops, a valve opens and the tube drains. When the meniscus reaches a second sensor, the.Passes an optical sensor that activates a timer. When solvent enters the tube, the meniscus.Here is the alphabet sequence of operation that typically This enables the testing solvent to be collected via a tube from the pump system into the FlowCal 5000 for measuring then directed into in a bottle for later disposal. Our example is the 12-oz, compact portable FlowCal 5000 digital flow meter placed at the end of HPLC pump systems being tested. How a Digital Flow Meter Worksįinally we get to the topic of this post. Replacing these time-consuming error-prone manual methods is accomplished with digital liquid flow meters such as the FlowCal 5000 offered by Tovatech. The service representative noted that it takes about 20 minutes to do a gravimetric test. A pharmaceutical industry service supplier tests dozens of HPLC pump systems at several locations. One user at a pharmaceutical company reported that they have 100 HPLC systems in their facility.

Performed but using a precision scale or analytical balance to weigh the Imagine, if you will, a lab with several HPLC pumpįor gravimetric testing similar exercises are Solvent evaporation can lead to measuring Until a standard deviation can be calculated. This tedious but critical process repeats over and over When sufficient volume isĬollected, the flow and stop watch are stopped and the flow rate in milliliters Measurement, testing technicians begin to collect HPLC pump system solvent in a graduatedĬylinder and simultaneously start the stopwatch. Part of the technician doing the testing. Include a stopwatch, excellent eyesight and a high degree of patience on the Gravimetric (requiring a precision analytical scale)īut the weak point, as we see it at Tovatech, is that both requirements.Volumetric (requiring a finely graduated cylinder) or.Manual alternatives for checking these systems are still Traditional Methods Measuring HPLC Pump System Accuracy

Into play and why they should be considered versus traditional methods, which This demands care toĪvoid or at least minimize wear on HPLC pump system components.īut equipment wear does occur and accuracy impacted meaning Rate accuracy such as 0.05 to 25 milliliters per minute. Complicating things is that these pump systems areĬomplex devices that include parts prone to leaks and wear when particulatesįurther complicating the topic, we are dealing with flow FlowCal 5000 Digital HPLC Liquid Flow MeterĪs suggested above, meeting regulations (and protecting a

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